The method of claim 1 or 2, wherein the mannosidase enzymatic activity is capable of hydrolyzing in vivo both Manα1,3 and Manα1,6 linkages of an oligosaccharide substrate comprising a Manα1,3 and Manα1,6 glycosidic linkage.Ħ. The method of claim 2, wherein the desired N-glycan is characterized as having at least the oligosaccharide branch Manα1,3 (Manα1,6) Manβ1,4-GlcNAc β1,4-GlcNAc-Asn.ĥ. The method of claim 2, wherein the desired N-glycan produced is selected from the group consisting of Man 3GlcNAc 2, GlcNAcMan 3GlcNAc 2 and Man 4GlcNAc 2.Ĥ. A method for producing a desired N-glycan in a lower eukaryotic host cell comprising the step of expressing in the cell a mannosidase enzymatic activity that is capable of hydrolyzing in vivo an oligosaccharide substrate comprising either or both a Manα1,3 and Manα1,6 glycosidic linkage wherein the desired N-glycan is produced within the host cell at a yield of at least 10 mole percent.ģ. A method for producing a human-like glycoprotein in a lower eukaryotic host cell comprising the step of expressing in the cell a mannosidase enzymatic activity that is capable of hydrolyzing an oligosaccharide substrate comprising either or both a Manα1,3 and Manα1,6 glycosidic linkage to the extent that at least 10% of the Manα1,3 and/or Manα1,6 linkages of the substrate are hydrolyzed in vivo.Ģ. SlpA as a tool for recombinant protein and enzyme technologyġ. Product and method for treatment of conditions associated with receptor-desensitization IDENTIFICATION OF PROTEIN ASSOCIATED WITH HEPATOCELLULAR CARCINOMA, GLIOBLASTOMA AND LUNG CANCERĪNTI-NOTCH3 AGONIST ANTIBODIES AND THEIR USE IN THE TREATMENT OF NOTCH3-RELATED DISEASESīreaking immunological toterance with a genetically encoded unnatural amino acid Suspension for the Generation of a Current of Electrons and the Use and the Preparation Thereof Methods for treating insulin resistance and identifying patients at risk for the disease Inhibition therapy for septic shock with mutant CD14
Gentle Process For Conversion of Cystine in Keratin-Containing Materials to Lanthionine